Published Work

Check out our latest preprints and publications...

Rapid design and prototyping of biocatalytic virus-like particle nanoreactors.

ACS Synth. Biol. 11, 2709-2718 (2022)

Pore structure controls stability and molecular flux in engineered protein cages
(with Lau Group USyd)

Science Advances, 8: eabl7346.

(2022)

Artificial Self-assembling Nanocompartment for Organizing Metabolic Pathways in Yeast

ACS Synth. Biol. 10, 3251–3263 (2021)

The structure of a plant-specific partitivirus capsid reveals a unique coat protein domain architecture with an intrinsically disordered protrusion

Communications Biology, volume 4, 1155 (2021)

All of Frank's and Sainsbury Group's publications

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ORCiD

Only in preprint:

Harding MD, Jackson MA, Gilding EK, Yap K, Craik DJ, Sainsbury F*, Lawrence N* (2024) A protein cage system for producing bioactive peptides in Escherichia coli. bioRxiv doi: 10.1101/2024.12.16.628579

Published:

Pande DC, Vu TH, Lu Y, Sainsbury F, Dau VT, Rehm BHA (2024) Restructuring Biologically Assembled Binding Protein-Biopolymer Conjugates toward Advanced Materials. ACS Applied Materials and Interfaces, 16: 68983-68995.

Sallam M, Mohammadi M, Sainsbury F, Nguyen NT, Kimizuka N, Muyldermans S, Benesova-Schafer M (2024) Bibliometric and scientometric analysis of PSMA-targeted radiotheranostics: knowledge mapping and global standing. Frontiers of Oncology 14:1397790.

Sallam M, Nguyen NT, Sainsbury F, Kimizuka N, Muyldermans S, Benesova-Schafer M (2024) PSMA-targeted radiotheranostics in modern nuclear medicine: then, now, and what of the future? Theranostics, 14:3043-3079.

Cheah LC, Sainsbury F, Vickers CE (2024) Translational fusion of terpene synthases for metabolic engineering: Lessons learned and practical considerations. Methods in Enzymology, 699:121-161.

Esquirol L, Newman J, Nebl T, Scott C, Vickers C, Sainsbury F, Peat TS (2024) Characterization of novel mevalonate kinases from the tardigrade Ramazzottius varieornatus and the psychrophilic archaeon Methanococcoides burtonii. Acta Crystallographica: D80, 203-215.

Cheah LC, Liu L, Plan MR, Peng B, Lu Z, Schenk G, Vickers CE*, Sainsbury F* (2023) Product profiles of promiscuous enzymes can be altered by controlling in vivo spatial organization. Advanced Science, 10: 2303415.

Seitz I, Saarinen S, Kumpula E-P, McNeale D, Anaya-Plaza E, Lampinen V, Hytönen VP, Sainsbury F, Cornelissen JJLM, Linko V, Huiskonen JT, Kostiainen MA (2023) DNA-origami-directed virus capsid polymorphism. Nature Nanotechnology, 18: 1205-1212.

McNeale D, Esquirol L, Okada S, Strampel S, Dashti N, Rehm B, Douglas T, Vickers CE Sainsbury F* (2023) Tunable In Vivo Co-localisation of Enzymes Within P22 Capsid-Based Nanoreactors. ACS Applied Materials and Interfaces, 15: 17705–17715.

Cheah LC, Liu L, Stark T, Plan MR, Peng B, Lu Z, Schenk G, Sainsbury F*, Vickers CE* (2023) Translational fusion of terpene synthases enhances metabolic flux by increasing protein stability. Metabolic Engineering, 77: 143-151.

McNeale D, Dashti N, Cheah LC, Sainsbury F* (2023) Protein Cargo Encapsulation by Virus-Like Particles: Strategies and Applications. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, e1869.

Esquirol L, McNeale D, Douglas T, Vickers CE, Sainsbury F* (2022) Rapid assembly and prototyping of biocatalytic virus-like particle nanoreactors. ACS Synthetic Biology, 11: 2709-2718.

Abidin RS, Sainsbury F* (2022) Bioengineering and Bioprocessing of Virus-Like Particle Vaccines in Escherichia coli. In: Rehm, B.H.A., Wibowo, D. eds Microbial Production of High-Value Products. Microbiology Monographs, vol 37. Springer, 271-288.

Adamson LSR, Tasneem N, Andreas MP, Close W, Szyszka TN, Jenner E, Young R, Cheah LC, Norman A, Sainsbury F, Giessen TW, Lau YH (2022) Pore structure controls stability and molecular flux in engineered protein cages.

Science Advances, 8: eabl7346.

Yeh C-M, Jarrett T, Gao Y, Zhao C-X, Whittaker A, Sainsbury F*, White A* (2022) Templating core-shell particles using metal ion-chelating biosurfactants. Particuology, 64: 145-152.

Cheah LC, Stark T, Adamson LSR, Abidin RS, Lau YH, Sainsbury F*, Vickers CE* (2021) An artificial self-assembling nanocompartment for organising metabolic pathways in yeast. ACS Synthetic Biology, 10: 3251-3263. Cover article

Byrne MJ, Kashyap A, Esquirol L, Ranson NA, Sainsbury F* (2021) Structure of a Plant-Specific Partitivirus Capsid Reveals a Novel Coat Protein Architecture with a Hypervariable Protrusion. Communications Biology, 4:1155.

Gao Y, Zhao C-X, Sainsbury F* (2021) Droplet Shape Control Using Microfluidics and Designer Biosurfactants. Journal of Colloid and Interface Science 584: 528–538.

Steinmetz NF, Lim S, Sainsbury F* (2020) Protein cages and virus-like particles: from fundamental insight to biomimetic therapeutics. Biomaterials Science 8, 2771-2777.

Sainsbury F* (2020) Emergence by design in self-assembling protein shells. ACS Nano 14: 2565-2568.

Jutras PV, Sainsbury F, Goulet MC, Lavoie PO, Tardif R, Hamel LP, D’Aoust MA, Michaud D (2020) pH Gradient Mitigation in the Leaf Cell Secretory Pathway Attenuates the Defense Response of Nicotiana benthamiana to Agroinfiltration. Journal of Proteome Research 19: 106-118.

Sainsbury F* (2020) Innovation in plant-based transient protein expression for infectious disease prevention and preparedness. Current Opinion in Biotechnology 61: 110-115.

Goulet MC, Sainsbury F, Michaud D (2020) Cystatin Activity–Based Protease Profiling to Select Protease Inhibitors Useful in Plant Protection. In JV Jorrin-Novo, L Valledor, MA Castillejo, M-D Rey, eds, Methods in Molecular Biology, Plant Proteomics: Methods and Protocols. Humana Press, NY, 353-366

Dashti NH, Sainsbury F* (2020) Virus-derived nanoparticles. In JA Gerrard, L Domigan, eds, Methods in Molecular Biology, Protein Nanotechnology. Humana Press, NY, pp 149-162.

Tayeb HH, Stienecker M, Middelberg A, Sainsbury F* (2019) Impact of Bioconjugation on the Interfacial Activity of a Protein Biosurfactant. Langmuir 35: 13588-13594.

Dashti NH, Abidin RS, Sainsbury F* (2018) Programmable in vitro co-encapsidation of guest proteins for intracellular delivery by virus-like particles. ACS Nano 12(5):4615-4623.

Jutras PV, Goulet MC, Lavoie PO, D'Aoust MA, Sainsbury F, Michaud D (2018) Recombinant protein susceptibility to proteolysis in the plant cell secretory pathway is pH‐dependent. Plant Biotechnology Journal 16: 1928-1938.

Yamada M, Tayeb H, Wang H, Dang N, Mohammed YH, Osseiran S, Belt PJ, Roberts MS, Evans CL, Sainsbury F*, Prow T* (2018) Using elongated microparticles to enhance tailorable nanoemulsion delivery in excised human skin and volunteers. Journal of Controlled Release 288: 264-276.

Sun B, Wibowo D, Sainsbury F, Zhao C-X (2018) Design and production of a novel antimicrobial fusion protein in Escherichia coli. Applied Microbiology and Biotechnology 102: 8763-8772.

Tasker A, Sainsbury F, Puttick S (2018) Particle-stabilised fluid-fluid interfaces: The impact of core composition on interfacial structure. Frontiers in Chemistry 6: 383.

Tayeb HH, Sainsbury F* (2018) Nanoemulsions in drug delivery: Formulation to medical application. Nanomedicine 13: 2525-2544.

Tayeb HH, Piantavigna S, Howard CB, Nouwens A, Mahler SM, Middelberg APJ, He L, Holt SA, Sainsbury F* (2017) Insights into the interfacial structure-function of poly(ethylene glycol)-decorated peptide-stabilised nanoscale emulsions. Soft Matter, 13: 7953-7961.

Brillault L, Jutras PV, Dashti N, Thuenemann EC, Morgan G, Lomonossoff GP, Landsberg MJ, Sainsbury F* (2017) Engineering recombinant virus-like nanoparticles from plants for cellular delivery. ACS Nano, 11: 3476-3484.

Rasoolizadeh A, Munger A, Goulet M-C, Sainsbury F, Cloutier C, Michaud D (2016) Functional proteomics-aided selection of protease inhibitors for herbivore insect control. Scientific Reports 6: 38827.

Sainsbury F, Jutras PV, Vorster J, Goulet M-C, Michaud D (2016) A chimeric affinity tag for efficient expression and chromatographic purification of heterologous proteins from plants. Frontiers in Plant Science 7: 141.

Rasoolizadeh A, Goulet M-C, Sainsbury F, Cloutier C, Michaud D (2016) Single substitutions to closely related amino acids contribute to the functional diversification of an insect-inducible, positively selected plant cystatin. FEBS Journal 283: 1323-1335.

Robert S, Jutras PJ, Khalf M, D’Aoust M-A, Goulet M-C, Sainsbury F, Michaud D (2016) Companion protease inhibitors for the in situ protection of recombinant proteins in plants. In R Menassa, J MacDonald, eds, Methods in Molecular Biology, Recombinant Proteins from Plants. Humana Press, NY, pp 115-126.

Saxena P, Thuenemann EC, Sainsbury F*, Lomonossoff GP (2016) Virus-based vectors for the expression of multiple proteins in plants. In R Menassa, J MacDonald, eds, Methods in Molecular Biology, Recombinant Proteins from Plants. Humana Press, NY, pp 39-54.

Abidin RS, Lua LHL, Middelberg APJ, Sainsbury F* (2015) Insert engineering and solubility screening improves recovery of virus-like particle subunits displaying hydrophobic epitopes. Protein Science 24: 1820-1828.

Robert S, Goulet M-C, D’Aoust M-A, Sainsbury F, Michaud D (2015) Leaf proteome rebalancing in Nicotiana benthamiana for upstream enrichment of a transiently expressed recombinant protein. Plant Biotechnology Journal 13: 1169-1179.

Vorster J, Rasoolizadeh A, Goulet M-C, Cloutier C, Sainsbury F, Michaud D (2015) Positive selection of digestive Cys Proteases in herbivorous Coleoptera. Insect Biochemistry and Molecular Biology 65: 10-19.

Catrice EVB, Sainsbury F* (2015) Assembly and purification of polyomavirus-like particles from plants. Molecular Biotechnology 57: 904-913.

Jutras PV, D’Aoust M-A, Couture M, Vezina L-P, Goulet M-C, Michaud D, Sainsbury F* (2015) Modulating secretory pathway pH by proton channel co-expression can increase recombinant protein stability in plants. Biotechnology Journal 10: 1478-1486.

Tekewe A, Connors NK, Sainsbury F, Wibowo N, Lua LHL, Middelberg APJ (2015) A rapid and simple screening method to identify conditions for enhanced stability of modular vaccine candidates. Biochemical Engineering Journal 100: 50-58.

Sainsbury F, Lomonossoff GP (2014) Transient expressions of synthetic biology in plants. Current Opinion in Plant Biology 19: 1-7.

Sainsbury F, Zeng BJ, Middelberg APJ (2014) Towards designer nanoemulsions for precision delivery of therapeutics. Current Opinion in Chemical Engineering 4: 11-17.

Lua LHL, Connors NK, Sainsbury F, Chuan YP, Wibowo N, Middelberg APJ (2014) Bioengineering virus-like particles as vaccines. Biotechnology and Bioengineering 111: 425-440.

Sainsbury F, Saxena P, Aljabali AAA, Saunders K, Evans DJ, Lomonossoff GP (2014) Genetic engineering and characterisation of Cowpea mosaic virus empty virus-like particles. In B Lin, B Ratna, eds, Methods in Biotechnology, Virus Hybrids as Nanomaterials: Methods and Protocols. Humana Press, NY, pp 139-153.

Sainsbury F, Varennes-Jutras P, Goulet M-C, D'Aoust M-A, Michaud D (2013) Tomato cystatin SlCYS8 as a stabilizing fusion partner for human serpin expression in plants. Plant Biotechnology Journal 11: 1058-1068.

Geisler K, Hughes RK, Sainsbury F, Lomonossoff GP, Rejzek M, Fairhurst S, Olsen CE, Motawia MS, Melton R, Hemmings AM, Bak S, Osbourn AE (2013) Biochemical analysis of a multi-functional cytochrome P450(CYP51) enzyme required for synthesis of antimicrobial triterpenes in plants. PNAS 110: E3360-E3367.

Robert S, Khalf M, Goulet M-C, D’Aoust M-A, Sainsbury F*, Michaud D* (2013) Protection of recombinant mammalian antibodies from development-dependent proteolysis in leaves of Nicotiana benthamiana. PLoS ONE 8(7): e70203, doi:10.1371/journal.pone.0070203.

Sainsbury F, Rhéaume A-J, Vorster BJ, Goulet M-C, Michaud D (2012) Discrimination of differentially inhibited cysteine proteases by activity-based profiling using cystatin variants with tailored specificities. Journal of Proteome Research 11: 5983-5993.

Munger A, Coenen K, Cantin L, Goulet C, Vaillancourt L-P, Goulet M-C, Tweddell R, Sainsbury F, Michaud D (2012) Beneficial pleiotropic effects of a cereal cystatin in transgenic potato lines. BMC Plant Biology 12: 198.

Sainsbury F, Benchabane M, Goulet M-C, Michaud D (2012) Multimodal protein constructs for herbivore insect control. Toxins 4: 455-475.

Goulet C, Khalf M, Sainsbury F, D’Aoust M-A, Michaud D (2012) A protease activity-depleted environment for heterologous proteins migrating towards the leaf cell apoplast. Plant Biotechnology Journal 10: 83-94.

Sainsbury F, Saxena P, Geisler K, Osbourn AE, Lomonossoff GP (2012) Using a virus-derived system to manipulate plant natural product biosynthetic pathways. Methods in Enzymology 517: 185-202.

Miyamoto K, Shimizu T, Lin F, Sainsbury F, Thuenemann E, Lomonossoff GP, Nojiri H, Yamane H, Okada K (2012) Identification of an E-box motif responsible for the expression of jasmonic acid-induced chitinase gene OsChia4a in rice. Journal of Plant Physiology 169: 621-627.

Vardakou M, Sainsbury F, Rigby N, Mulholland F, Lomonossoff GP (2012) Expression of active recombinant human gastric lipase in N. Benthamiana using the CPMV-HT transient expression system. Protein Expression and Purification 81: 69-74.

Sainsbury F, Saunders K, Aljabali AAA, Evans DJ, Lomonossoff GP (2011) Peptide-controlled access to the interior surface of empty virus nanoparticles. ChemBioChem 12: 2435-2440.

Saxena P, Hsieh Y-C, Alvarado VY, Sainsbury F, Saunders K, Lomonossoff GP, Scholthof HB (2011) Improved foreign gene expression in plants using a virus-encoded suppressor of RNA silencing modified to be developmentally harmless. Plant Biotechnology Journal 9: 703-712.

Sainsbury F*, Sack M, Stadlmann J, Quendler H, Fischer R, Lomonossoff GP (2010) Rapid transient production in plants by replicating and non-replicating vectors yields high quality functional anti-HIV antibody. PLoS ONE 5(11): e13976.

Sainsbury F, Cañizares MC, Lomonossoff GP (2010) Cowpea mosaic virus: the plant virus-based biotechnology workhorse. Annual Review of Phytopathology 48: 437-455.

Aljabali AAA, Sainsbury F, Lomonossoff GP, Evans DJ (2010) Cowpea mosaic virus unmodified empty virus-like particles can be loaded with metal and metal oxide. Small 6: 818-821.

Sainsbury F, Theunemann EC, Lomonossoff GP (2009) pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants. Plant Biotechnology Journal 7: 682-693.

Saunders K, Sainsbury F, Lomonossoff GP (2009) Efficient generation of Cowpea mosaic virus empty virus-like particles by the proteolytic processing of precursors in insect cells and plants. Virology 393: 329-337.

Mugford ST, Qi X, Bakht S, Hill L, Wegel E, Hughes RK, Papadopoulou K, Melton R, Philo M, Sainsbury F, Lomonossoff GP, Roy AD, Goss RJM, Osbourn AE (2009) A serine carboxypeptidase-like acyltransferase is required for synthesis of antimicrobial compounds and disease resistance in oats. Plant Cell 21: 2473-2484.

Sainsbury F, Liu L, Lomonossoff GP (2009) Cowpea mosaic virus-based expression of antigens and antibodies in plants. In L Faye, V Gomord, eds, Methods in Biotechnology, Recombinant Pharmaceutical Proteins from Plants. Humana Press, NY, pp 25-39.

Sainsbury F, Lomonossoff GP (2008) Extremely high-level and rapid transient protein production in plants without the use of viral replication. Plant Physiology 148: 1212-1218.

Sainsbury F, Lavoie P-O, D'Aoust M-A, Vezina L-P, Lomonossoff GP (2008) Expression of multiple proteins using full-length and deleted versions of Cowpea mosaic virus RNA-2. Plant Biotechnology Journal 6: 82-92.

Sainsbury F, Collings DA, Mackun K, Gardiner J, Harper JDI, Marc J (2008) Developmental reorientation of transverse cortical microtubules to longitudinal directions: a role for actomyosin-based streaming and partial microtubule-membrane detachment. Plant Journal 56: 116-131.

Sainsbury F, Cañizares MC, Lomonossoff GP (2007) Cowpea mosaic virus-based expression vectors. In K Hefferon, ed, Virus Expression Vectors Transworld Research Network, Kerala, India, pp 339-555.